【摘要】 目的　探讨结直肠癌 (CRC)患者的组织与粪便中K ras癌基因 12位点突变检测结果的联系及检出率差异 ,为建立结直肠癌早期筛检方法提供科学依据。方法　应用聚合酶链反应 限制性片段长度多态性分析 (PCR RFLP)方法对 45例原发性结直肠癌患者手术切除的组织及术前粪便标本中K ras癌基因 12位点的突变情况进行检测 ,结果采用卡方检验及卡柏值 (Kappaval ue)进行统计分析。结果　癌组织、远端手术切缘粘膜、粪便标本中的K ras癌基因 12位点突变检出率分别为 3 5 .5 6% (16/45 )、4.44% (2 /45 )、3 7.78% (17/45 )。癌组织与粪便标本检测结果的关联检验为 :χ2 =3 3 .0 9,P <0 .0 1;差别检验为 :χ2 =0 ,P >0 .0 5 ;观察一致率为 93 .3 3 % ,卡柏值为 0 .86。结论　粪便中K ras癌基因 12位点突变检测结果与癌组织的检测结果存在联系 ,其检出率无显著性差异 ,可靠性极好 ;且粪便取材方便 ,无创伤性 ,可能作为结直肠癌高危人群的筛检方法更多还原

【Abstract】 Objective In order to set up a quick and simple method for early diagnosis of colorectal cancer(CRC).Methods The K-ras oncogene codon 12 mutation in surgically resected tissue specimens and stool samples from 45 patients with CRC were examined by PCR-RFLP,then the results were tested with kappa value and chi-square test of pair data.Results Positive rate of tumor tissues and stool samples was 35.56%(16/45),4.44%(2/45) and 37.78(17/45),respectively.The test of statistic relationshipby chi-square test of pair data was χ2=33.09 and P<0.01,the test of statistic difference was χ2=0 and P>0.05,the observational agreement was 93.33% and Kappa value was 0.86.Conclusion The detection results of K-ras oncogene codon 12 mutation in the stools is related with that in the tumor tissues.There is no statistic difference between the positive rate of the tumor tissues and stools.The reliability is very good.Moreover,because of the convenience and noninvasiveness,this method can be used to screen CRC in high risk population.更多还原