【摘要】 目的　研究核转录因子NF -κΒ在大鼠肝脏缺血再灌注损伤中的作用。方法　采用快速提取法提取肝组织中细胞核蛋白 ,再用凝胶滞留电泳分析测定核转录因子NF -κΒ与其靶基因特异DNA序列的结合活性。结果　肝脏缺血再灌注时 ,NF -κΒ与其特异调节基因序列的结合活性明显升高 ,且有一定的时间相关性。结论　说明NF -κΒ与其特异调节基因序列的结合活性与肝脏缺血再灌注时肝损伤程度有直接关系。更多还原

【Abstract】 Objective To determine hepatic ischemia/reperfusion induced NF kappa B activation. Methods Rats underwent partial hepatic ischemia and reperfusion.The NF kappa B activation was evaluated. Results Hepatic NF kappa B activation was induced in a time dependent fashion. Conclusion The data suggest that hepatic ischemia/reperfusion injury is related to the binding activation of NK kappa B to special DNA sequence.更多还原