【摘要】 对三种不同培养方法进行细胞生长速度、密度、营养及代谢产物浓度的比较分析 ,以优化和筛选最佳培养条件与方式。用同体积生物反应罐 ,基本培养条件相同 ,采用批培养、再循环培养、灌流培养三种方式进行了Vero细胞微载体 (CytodexI)的周期培养。三种培养方法均达到预期效果 ,最终细胞密度分别为每毫升 2 .0 9× 10 6 、3 .3 7×10 6 、4 .3 4× 10 6 。灌流培养倍增水平最高 ( 5 .5 9) ,倍增时间最少 ( 3 0 .0 5h)。表明灌流培养优于再循环培养 ;而再循环培养兼容批培养及灌流培养的特点 ,又优于批培养。更多还原

【Abstract】 Vero cells were cultivated periodically on microcarriers Cytodex I by using batch culture mode, recirculation culture mode and perfusion culture mode, respectively in the bioreactor of the same volume 7L and under the basic cultivation conditions. Expected result were obtained for three culture modes. Their final cell densities were: 2.09×10 6 cells/ml; 3.37×10 6 cells/ml; 4.34×10 6cells/ml; while the perfusion cultures doubling level was the highest of 5.59; the doubling time was the least of 30.05 hour. The experimental data indicated that the perfusion culture, was excelled than that of the recirculation culture, and that the recirculation culture which had the characteristics of both perfusion and batch cultures was excelled than that of the batch culture.更多还原