【摘要】 使用硫酸铵分级沉淀、DEAE -TOYOPEARL 6 5 0M和POROS 2 0PI弱阴离子交换色谱等分离技术 ,从黑曲霉 (Aspergillusniger)发酵酶粉中分离提纯出一种新内切 β -葡聚糖苷酶 ,在非还原条件下分子量为 36kD ,还原条件为 38kD ,该酶最适pH3 5 ,最适温度 5 5℃ ,N -末端部分氨基酸序列为XXXFKCVGSMEDGAES。更多还原

【Abstract】 A novel cellulase was identified and isolated from a commercial Aspergillus niger cellulase preparation.The crude enzyme preparation,which was prepared by precipitation of the water extract of the culture of Aspergillus niger with ammonium sulfate,was further fractionated by three steps of chromatography of weak anion exchange to obtain an electrophoretically homogeneous cellulase. The molecular weight of the purified enzyme was estimated by SDS-PAGE to be 36kD under non-reduced conditions and 38kD under reduced conditions, with the optimun pH at 3.5, and the optimum temperature at 55℃. The partial N-terminal amino acid sequences of the purified enzyme was identified as XXXFKCVGSMEDGAES.更多还原