【摘要】 设计并完成了 3种水稻线粒体tRNATrp的突变 ,体外转录并用枯草杆菌和人色氨酰tRNA合成酶 (TrpRS)对tRNATrp及其突变体进行了活力测定 .3种突变体的氨酰化活力比野生型水稻线粒体tRNATrp分别上升了 1 8、1 5和 5倍 .说明A1 U72和G5 C68对于提高线粒体tRNATrp被细胞质TrpRS氨酰化能力的作用并不大 ,细胞质tRNATrp与细胞质TrpRS的识别方式并不适用于线粒体tRNATrp与细胞质TrpRS的相互识别 .研究结果对于了解线粒体tRNATrp和细胞质TrpRS的相互识别及药物设计有重要意义更多还原

【Abstract】 Three mutants from Oryza sativa mitochondrial tRNA Trp were constructed. In vitro transcripts of these mitochondrial tRNA Trp genes were tryptophanylated by Bacillus subtilis tryptophanyl\|tRNA synthetase(TrpRS)and Homo sapiens TrpRS.The aminoacryl activities of these 3 mutants were 1.8,1.5 and 5 times higher than the wild\|type Oryza sativa mitochondrial tRNA Trp .The results indicated that A1\|U72 and G5\|C68 base pairs were not dominant to the aminoacryl activity.The recognition mechanism of cytoplasmic tRNA Trp and TrpRS was not suitable for mitochondrial tRNA Trp and cytoplasmic TrpRS.This work was important to drug devlopment and investigating the recognition mechanism of mitochondrial tRNA Trp and cytoplasmic TrpRS.更多还原