【摘要】 分别以两类沙田柚侧芽为接称、酸柚为砧木进行了试管嫁接，结果表明：不同嫁接方式、接穗大小以及不同激素处理均直接影响到试管嫁接成活率。对大田侧芽而言，接穗大小以茎尖为宜，１．０ｍｇ／ＬＧＡ３或０．５ｍｇ／Ｌ６-ＢＡ处理效果较佳，嫁接成活率分别达５０％和３０％；对侧枝沙培萌发的侧芽而言，接穗大小以茎尖＋１个节间为宜，２．０ｍｇ／Ｌ ＧＡ３或０．５ｍｇ／Ｌ６-ＢＡ处理效果较佳，嫁接成活率分别达９０％和８０％。在相同条件下，后者的嫁接成活率一般明显高于前者。两种沙田柚嫁接苗的试管微繁殖没有明显区别，在ＭＳ＋６－ＢＡ ０．１ｍｇ／Ｌ培养基上均可分化不定芽，继代的嫁接成活率可达９０％以上。更多还原

【Abstract】 We carded out the tube-grafting with two kinds of lateral shoots of Shatian pomelo as scions and acid pommelo as stock separately, it indicated different grafting-modes, the sizes of scion and the treatments with different hormones could all affect the survival percentage of tube-grafting. The lateral shoots coming from field: the shoot -tip alone was optimal as the scion, and the effect was better when treated with 1.0 mg/L GA3 or 0.5 mg/L 6-BA, the survival percentage got 50% and 30% respec- tively. The lateral shoots coming from lateral branch with sand-culture medium: the shoot-tip with one node was optimal as the scion, and the effect was better when treated with 2.0 mg/L GA3 or 0.5 mg/ L 6 -BA, survival percentage got 90% and 80% respectively. The survival percentage of grafting of the latter was much higer commonly than the former in same condition. There were not obvious differ- ences on the micropropagation of the two kinds of grafting-plantlets, they could all differentiate the ad- ventitious shoots in MS + 6 -BA 0.1 mg/L, the survival percentage of next era got over 90%.更多还原