【摘要】 苹果梨多酚氧化酶仅作用于邻苯酚,与对苯酚、间苯酚和一元酚无作用。以儿茶酚为底物,该酶的最适pH为6.5,在30℃下活性最强。除氯化钠和蔗糖外,供试的其它7种抑制剂均可不同程度地对该酶产生抑制,其中以焦亚硫酸钾效果最好。更多还原

【Abstract】 Polyphenoloxidase in Pinguoli pear(Pyrus bretschneideri Rehder:Pinguoli)showed reaction with odiphenol,but not with hyroguinone,m-diphenol,and mono-phenol.Maximum PPO activities showed pH 6.5,and optimum temperature for maximum PPO activity was 30℃ with catechol as a substrate.The enzyme was relatively sensitive to most inhibiors tested with the exception of NaCl and sucrose.Potassium pyrasufite was the most effective inhibitor.更多还原