【摘要】 摸索了 2709碱性蛋白酶水解酪蛋白制备 CPP的最佳工艺条件：底物浓度 10％、用酶量 1500μ /g反应温度 45℃、 pH10.5、反应时间 150min。采用选择沉淀法分离 CPP，在 pH4.5添加 1.1％ CaCl 2（ w/v）和 50％（ v/v）乙醇，室温沉淀 4h，得到 CPP产品的 N/P、得率分别为 6.39％和 13.94％。更多还原

【Abstract】 In this study,alcalase was used for producing CPP by hydrolyzing casein.The optimum conditions:casein concentration 10％ ,enzyme usage 1500u/g substrate,temperature 45℃ ,pH10.5 and time 150mins were determined.A selective precipitation procedure was also used to isolate CPP from the hydrolyzate of casein.The N/P ratio and the yield of CPP obtained by adding 1.1％ CaCl 2(w/v) and 50％ (v/v) alcohol to the hyrolyzate at pH4.5 were 6.39％ and 13.94％ respectively.更多还原