【摘要】 采用原位杂交、免疫荧光双标技术及大鼠创伤应激模型，观察孤啡肽对海马白介素－１β（ＩＬ－１β）表达水平的影响。结果显示，侧脑室注射抗大鼠ＩＬ－１β抗体能明显改善创伤介导的免疫反应，即有效下调巨噬细胞分泌ＩＬ－１和ＴＮＦ－α的能力。侧脑室注射孤啡肽后２ｈ海马ＩＬ－１β的表达明显下降，且此作用能被孤啡肽受体拮抗剂阻断；免疫荧光双标技术结合激光共聚焦显微镜观察发现，孤啡肽的受体在神经元、星形胶质细胞及小胶质细胞均有表达。实验结果提示，海马的ＩＬ－１β参与创伤应激介导的免疫反应，孤啡肽的神经免疫调节作用可能是通过作用于海马的中枢神经细胞，抑制ＩＬ－１β的合成及释放而完成的。更多还原

【Abstract】 Using in situ hybridization and immuno-fluorescent double labeling method, we investigated the function of orphanin EQ on interlenkin-1β (IL-1β) transcripts in hippocampus. Intracerebroventricularly (icv) administrated IL-1β antibody reduced IL-1 and TNF-α secreted from peritoneal macrophage.The high level of IL-1β transcript in hippocampus elicited by traumatic stress was blocked by icy injection of orphanin EQ (0.55 nmol), which was reversed by orphanin EQ receptor antagonist ([phe1 (CH2-NH)Gly2]nociceptin-( 1-13)-NH2). Opioid receptor-like receptor transcripts were found in neuron, astrocyte and microglia. Based on the results, we conclude that orphanin EQ functions as a neuroimmune modulator, and provokes immune response via mediation of IL-1β derived from neuron, astrocyte and microglia in hippocampus.更多还原