【摘要】 目的分析我国荒漠、山丘疫区利什曼原虫分离株的 SSU r DNA多变区序列差异。方法 n DNA进行PCR扩增 ,将扩增出的 SSU r DNA基因的特异片段克隆于 p GEMR-T Easy Vector上 ,采用通用引物 M1 3进行 PCR扩增 ,全自动测序仪测序。结果序列分析显示本文报道的荒漠、山丘疫区的 2株利什曼原虫 (L.d.XJ771、L.d.SC6)的 SSU r DNA序列大小均为 3 92 bp;序列差异发生在两个独特序列区 (UQ- 和 UQ- ) ,无移码突变 ;与 Gene Bank中的利什曼原虫比较分析 ,同源性在 98%以上。结论我国荒漠、山丘疫区利什曼原虫分离株之间的 SSU r DNA多变区的碱基序列有差异 ;荒漠疫区分离株 L.d.XJ771与国际标准株 L.d.DD8的 SSU r DNA多变区的碱基序列完全相同更多还原

【Abstract】 Aim To analyze the sequence of the SSU rDNA variable region of Leishmania isolates from desert and hill foci of China. Methods Specific SSU rDNA fragments from nuclear DNA of two Leishmania isolates were amplified by PCR and then cloned into pGEMR-T Easy Vector.After that, the specific fragments were sequenced by the automated DNA sequencer. Results Sequence analysis showed that the amplified DNA fragments of two Leishmania isolates(L.d.XJ771 and L.d.SC6) were all 392bp in length, point mutations were located in the two unique sequence (UQ-Ⅰ and UQ-Ⅱ),and no insertion/deletion found. The identities of comparison of Leishmania in GeneBank were more than 98%.Conclusion Sequence difference of the SSU rDNA variable region existed among Leishmania isolates from desert and hill foci; The sequences of the SSU rDNA variable region of L.d.XJ771 isolate and L. d.DD8 were identical.更多还原