【摘要】 目的　研究蚯蚓毒素的来源及其成分 ,探索其去除方法。方法对蚯蚓应激分泌液进行透析、可见及紫外分光光度法检测透析袋内外液组分、电泳分析蛋白组分、动物体内试验检测毒性。结果蚯蚓应激分泌液的蛋白组分显示剧毒性 (最小致死量为 0 .2 42mg/kg)。PAGE分离主要有两条带 ;蚯蚓对不同刺激产生的应激分泌液成分相同。结论蚯蚓应激分泌液的蛋白组分有毒 ,用加盐或微电流刺激方法可去除毒素。更多还原

【Abstract】 PurposeThe source of earthworm toxin, its ingredients and the methods to take it off were studied.MethodsThe irritable secretor was dialyzed, and then examined by UV-Vis spectrum instrument.The proteins was analyzed with electrophoresis. The toxicity was tested in vivo.ResultsThe proteins of irritable secretor 0.242mg/kg iv cause a mouse to death.Many bands were separated from the proteins with SDS-PAGE, among which two kinds were the principal. Even with different stimulus the secretor of earthworm was identical in ingredients.ConclusionThe results demonstrated that the earthworm proteins of irritable secretion had toxicity and the toxin could be removed from earthworm by adding NaCl or 40V electricity.更多还原