【摘要】 目的　探讨GM CSF联合IL 4对髓性白血病细胞表面B7分子表达的上调作用。方法　从初诊急性或慢性髓性白血病患者的外周血中分离出单个核细胞 (PBMNC) ,用rhGM CSF联合rhIL 4共同孵育 7～ 10d ;通过流式细胞仪检测细胞因子诱导前后细胞表面B7分子的表达。结果　未经细胞因子诱导的白血病细胞表面B7分子低表达或缺如 ,经过GM CSF联合IL 47～ 10d的诱导 ,可显著上调B7分子的表达。结论　髓性白血病细胞表面存在B7分子表达缺陷 ;用GM CSF联合IL 4进行诱导是纠正这种缺陷的一条途径更多还原

【Abstract】 Purpose To investigate the upregulation efficacy of rhGM?CSF and rhIL?4 in B7 costimulatory molecules expression on myeloid leukemia cells. Methods MNCs were isolated from peripheral blood of acute and chronic myeloid leukemia patients,co cultured with rhGM?CSF and rhIL?4 for 7-10 days.Before and after cytokines induced,B7?1 (CD80) and B7?2 (CD86) expression was assayed by flow cytometry. Results Before induced,average B7?1 and B7?2 expression was (1.20±0.79)% and (3.69±2.23)%, respectively .After 7 days cytokines co cultured,average epression was (8.98±2.98)% and (22.24± 5.07 )%,respectively.And after 10 days co cultured,the expression was (6.58±3.11)% and (14.90± 5.73 )%,respectively. Conclusions B7 costimulatory molecules expression on the myeloid leukemia cell surface was defective.These costimulatory molecules on the myeloid leukemia cells can be upregulated by a combination of rhGM?CSF and rhIL?4.更多还原