【摘要】 用免疫荧光化学与激光共聚焦显微术结合的方法研究了小鼠卵母细胞中蛋白激酶C (PKC)α和 βⅠ的表达和定位 ,以及蛋白激酶C (PKC)和皮质颗粒的双标记 ,探讨了以哺乳动物卵母细胞为实验对象进行免疫荧光共聚焦显微研究的简便方法 .结果发现 ,PKCα和 βⅠ在小鼠生发泡期和MⅡ期卵母细胞中都有表达 ,但表达部位存在差异 .说明采用改进的激光共聚焦显微术 ,可以方便、灵敏地检测特异蛋白质在卵母细胞中的表达部位 ,从而为生殖、发育研究提供有效手段更多还原

【Abstract】 The expression and subcellular localization of protein kinase C alpha and bata Ⅰ in mouse oocytes were studied by immunofluorochemistry and confocal laser microscopy. Double staining was performed to detect the PKC and cortical granules of the MⅡ stage eggs. The results show that both PKCα and βⅠ were expressed in mouse oocyte at GⅤ and MⅡ stages, but their subcellular localization were different. Simple methods of immunofluorochemistry and confocal laser microscopy in mammalian oocyte were developed. By these methods, the expression and subcellular localization can be studied conveniently and precisely, so as to provide an effective tool to the research of reproductive and developmental biology.更多还原