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青海湖裸鲤血清淀粉酶的研究

Investigation on Blood Serum Amylase of Qinghai-lake Naked Carps

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【作者】 李太平赫广春赵凯吴华李军祥

【Author】 LI Tai-ping, HE Guang-chun, ZHAO Kai, WU Hua, LI Jun-xiang (Faculty of Agriculture and Animal Sciences, Qinghai University, Qinghai Xining 810003,China)

【机构】 青海大学农牧学院动物科学系!青海西宁810003青海省水产研究所!青海西宁810012

【摘要】 采用聚丙烯酰胺垂直电泳法对120尾青海湖裸鲤进行了血清淀粉酶(AMY)电泳观察。结果表明:青海湖裸鲤血清淀粉酶有AMY1、AMY2和AMY3三种。AMY2同工酶在个体间存在多态性,其酶型分别为AMY2AA型(5.8%)、AMY2BB型(10.8%)、AMY2AB型(13.3%)和AMY2O型(70.0%)。AMY1和AMY3显现出单态。用同样的方法研究了鲤鱼和草鱼血清淀粉酶,结果其多态性谱带与青海湖裸鲤非常相似。认为,血清淀粉酶(AMY)在不同亚科鱼类间差异可能不显著。

【Abstract】 s: 120 Qinghai-lake naked carps (Gymnocypris przewalskii Qinghai Lake Naked Craps)were used to investigate polymorphism of the blood serum amylase by means of polyacrylamide gel electrophoresis.The results were as follows: There were three isozymes including AMY1, AMY2, AMY3 in Qinghai-lake naked carps. There were four phenotypes including AMY2AA(5.8%),AMY2BB(10.8%), AMY2AB(13.3%) and AMY2O(70.0%) on the locus of AMY2, AMY1 and AMY3 were not found polymorphis existed.The analysis showed that the blood serum amylase could perhaps be species characteristics and not subfamily characteristics.

【基金】 国家自然科学基金(39860060);教育部“高等学校骨干教师资助计划”项目资助。
  • 【文献出处】 四川畜牧兽医 ,Sichuan Animal and Veterin Sciences , 编辑部邮箱 ,2001年09期
  • 【分类号】Q959.468
  • 【被引频次】4
  • 【下载频次】99
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