【摘要】 作者以卡特丽亚兰开花植株茎尖和试管苗茎段或叶切段为外植体 ,成功地诱导形成原球茎并获得再生植株 ,筛选出了原球茎球诱导培养基 :RE + 6mg/LBA (5mg/LBA + 0 .5～ 1mg/LNAA或 1BA) + 0 .5 %～ 1%蔗糖 ;原球茎增殖培养基 :MS(或KC) + 5mg/LBA + 0 .1(0 .5 )mg/LNAA(或 0 .5mg/L 1BA) + 0 .2 %蛋白胨和分化及育苗培养基 :KC + 0 .3mg/L(0 .5 )NAA + 10 %香蕉匀汁 + 0 .1%活性炭 .BA在原球茎的产生、增殖分化中起着主导作用 .更多还原

【Abstract】 The clonal propagation of Cattleya was developed.PLB is obtained through culture of leaf segments and shoot tips and shoot stem of Cattl eya on KC solid medium with BA 6mg/L, NAA 0.5%～lmg/L and sucrose 0.5%～1%. proliferation of Protocorms is on MS or KC medium with BA 5mg/L and NAA 0.l～0.5mg/L or IBA 0.5mg/L and Peptone 0.2%. The medium as KC or MS with NAA 0.3 or 0.5mg/L and 10% banana extracts and 0.l% actived charcoal is better for plantlet growth. BA played a central role in the protocorm formation and differentiation.更多还原