【摘要】 以抗旱性较强的荔枝东刘１号和抗旱性较弱的陈紫１—２年生盆栽实生幼苗为试验材料，研究了水分胁迫对荔枝（Ｌｉｔｃｈｉ ｃｈｉｎｅｎｓｉｓ Ｓｏｎｎ．）叶片细胞胞质以及以离子键和共价键与细胞壁结合的Ｈ＋－ＡＴＰａｓｅ活性的影响。结果表明：（１）叶片相对含水量随水分胁迫程度的增加而减少，抗旱性强的东刘１号下降的幅度小于抗旱性弱的陈紫。（２）Ｈ＋－ＡＴＰａｓｅ在细胞中的分布是：细胞胞质Ｈ＋－ＡＴＰａｓｅ占绝大多数，其次是共价键结合Ｈ＋－ＡＴＰａｓｅ，离子键结合Ｈ＋－ＡＴＰａｓｅ最少，品种间差异不明显。（３）在水分胁迫下，荔枝叶片Ｈ＋－ＡＴＰａｓｅ活性（比活性）均上升，抗旱性强的品种上升的幅度均大于抗旱性弱的品种。更多还原

【Abstract】 One to 2-year-old seedlings of two lychee (Litchi chinensis Sonn.) cultivars (droughtsensitive Chenzi and drought-resistant Dongliu 1) were planted in pots by moisture stress and normal watering to determine the relative water content (RWC) and the H+-ATPase activities in leaves. The effects of water stress on (specific) activity of cytoplasmic, ionically and covalently wall-bound H+-ATPase in leaves were studied. The results showed that RWC in leaves decreased with increasing water stress, but such decrease was less in droughtresistant cultivar than in drought-sensitive one. The activities of cytoplasmic, covalently wall-bound and ionically wall-bound H+-ATPase were 81.80% - 83.15%, 11.48% - 12.30% and 5.37%-5.90% of the total H+-ATPase activity, respectively. No significant difference was observed between two cultivars. Under water stress, the (specific) activities of Cytoplasmic, ionically wall-bound and covalently wall-bound H+-ATPase in leaves increased. The extent of increase was higher in drought-resistant cultivar than in drought-sensitive one.更多还原