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固定化活细胞发酵生产乳链菌肽
Nisin fermentation with immobilized growing cell
【摘要】 以卡拉胶为材料对乳酸乳球菌Y-72进行了固定化分批发酵和连续发酵的研究,测定了2种情况下发酵液效价、细胞游离数等参数。固定化分批发酵效价达到1 420 IU/mL,但发酵伴随着细胞的大量游离,在固定化连续发酵时则克服了这一缺点,该发酵连续运行144 h未见发酵液效价下降,发酵液效价稳定在600~800 IU/mL之间。
【Abstract】 :In this study,immobilized batch and continuous fermentation was carried out with Lactoccus lactis subsp.Lactis Y-72 which was immobilized by κ-carrageenan.In these two conditions many parameters were detected.It was founded that,in the immobilized batch process,the nisin activity can reach to 1 420 IU/mL,but with many cells released.In the continuous fermentation,this defect was avoided and this fermentation was performed for 144 h without the decrease of activity,the activity is between 600 and 800 IU/mL.
【关键词】 固定化活细胞;
乳链菌肽;
发酵;
卡拉胶;
【Key words】 immobilized growing cell; Nisin; fermentation; κ-carrageenan;
【Key words】 immobilized growing cell; Nisin; fermentation; κ-carrageenan;
- 【文献出处】 中国乳品工业 ,China Dairy Industry , 编辑部邮箱 ,2001年02期
- 【分类号】TS201.3
- 【被引频次】14
- 【下载频次】225