【摘要】 探讨了白菜材料类型、球型、熟性等不同遗传背景以及培养基中添加活性炭对小孢子胚胎发生能力（ＭＥＡ）的影响。结果表明：结球白菜比不结球白菜、结球白菜中的叠抱类型比合抱类型、早熟类型比中晚熟类型具有更高的ＭＥＡ。培养基中添加一定浓度的活性炭能明显促进培养小孢子的胚胎发生及发育，特别对低ＭＥＡ材料的促进作用更为显著。更多还原

【Abstract】 It is well known that genotype, growth environments of donor plants and culture conditions(medium composition and temperature etc.)are main factors influencing embryogenesis of isolated microspores in Brassica crops.To get maximum response of microspore embryogenesis in various genotypes and to utilize the microspore culture technique to commercial breeding programs in Chinese cabbage,more details relating microspore embryogenic ability(MEA)should be clarified.In present study,based on the culture results(percentage of response cultivars and embryo yields)of 106 genotypes,factors influencing MEA in Chinese cabbage were further discussed.In general,heading Chinese cabbage is more responsive than non-heading Chinese cabbage.In heading Chinese cabbage,early-mature varieties are more responsive than mid-and late-mature ones.Ratio of genotypes with high MEA is higher in flat-topped heading types than that in ovate types.Adding 0.7 mg/mL activated charcoal to the liquid culture medium showed obvious positive effects on formation and development of microspore embryos in Chinese cabbage,especially in low-responsive varieties.更多还原