【摘要】 本研究选取玉兰（Ｍａｇｎｏｌｉａ　ｄｅｎｕｄａｔａ　Ｄｅｓｒ．）为材料，提取总ＲＮＡ，利用设计的肌动蛋白编码区两端的特异性引物和５＇ＲＡＣＥ　试剂盒，扩增出肌动蛋白编码区的ｃＤＮＡ基因。以豌豆卷须肌动蛋白基因作探针，通过Ｓｏｕｔｈｅｒｎ杂交进行检测，证明同源性很高。将所获得序列克隆到ｐＧＥＭ－Ｔ载体上进行测序，测序结果在ＧｅｎＢａｎｋ中进行查询，并与其他高等植物肌动蛋白基因序列进行了分析。更多还原

【Abstract】 We devised a pair of primers according to actin genes coding region sequence.Total RNA was isolated from leaves of Magnolia.denudata Desr.,then a 5’RACE kit was employed to obtain actin cDNA gene.Southern blotting analysis showed that there is highly similarity between actin gene of M.denudata Desr.and the probe from Pea tendril＇s.The actin cDNA gene was sequenced by cloning into pGEM-T vector.Results demonstrated that the sequence is actin gene of M.denudata Desr.Its structural characterizations were discussed.更多还原