【摘要】 选择 2 0只体重相似的成年雌性SD大鼠 ,自然交配妊娠后随机分为试验组 (n =10 )和对照组 (n =10 ) ,分别在妊娠第4 ,10 ,13d给试验组母鼠静脉注射 5 0 μg·kg-1IGF 2片段 ,对照组注射等量生理盐水 ,于妊娠第 1,4 ,7,10 ,13,17,2 0d采集血样 母鼠自然分娩后 ,新生仔鼠称重 ,断头采血 检测血浆IGF 2及代谢物水平的变化 另外选择 2 5只成年SD妊娠 (试验组n =15和对照组n =10 ) ,母鼠进行皮下注射研究 ,注射时间为妊娠第 4 ,10和 17d ,其它试验步骤同上 结果表明 ,静脉注射IGF 2片段后 ,试验组母鼠血浆IGF 2水平高于对照组 2 7.14 % ,血糖水平显著下降 18.99% ;试验组新生仔鼠血浆IGF 2高于对照组 4 8.2 6 % ,窝产仔数及仔鼠均重较大 皮下注射后试验组新生仔鼠血浆IGF 2 ,产仔数和仔鼠窝重和均重都有所提高 ,但与对照组没有显著差异 说明给妊娠母鼠注射IGF 2片段可产生胰岛素样代谢效应 ,促进胚胎生长发育和提高其成活率 ,静脉注射较皮下注射的作用明显更多还原

【Abstract】 The study was conducted on pregnant rats to examine the effects of IGF 2 fragment on the metabolism both in mother and fetuses and on fetal growth. In the first experiment, 20 adult rats of certain gestation were put in two groups. 10 pregnant rats in IGF 2 treated group were injected in 50 μg·kg -1 of IGF 2 fragment on day 4,10 and 13 of gestation respectively, the other 10 rats in the control group were injected the same mamount of saline. Blood samples were taken on day 1,4,7,10,13,17 and 20 of gestation respectively. Upon the birth, the neonatal pups were weighed xand killed for taking blood sample and all blood samples of the same litter were pooled together. In the second experiment, 25 adult pregnant rats were used and also put in two groups as described above, 15 rats in the treated group were injected 50 μg·kg -1 of IGF 2 fragment subcutaneouslly on day 4,10 and 17 of gestation, others in the control group were treated as in the first experiment. The results showed that IGF 2 fragment iv injection raised maternal IGF 2 concentrations by 27.14%, and reduced maternal glucose by 18.99%. The neonatal pups in treated group showed a 48.26% higher of IGF 2 concentration than control, and the litter number and average weight of pups in treated rats were all higher than control. In experiment with IGF 2 fragment subcutaneous injection , plasma IGF 2, glucose and other metabolites presented no significant differences with control group, so did the reproductive performances. It is concluded that IGF 2 fragment injected iv can cause insulin like metabolic effects, and may stimulate the fetal growth and survival rate by elevating the IGF 2 levels.更多还原