【摘要】 以软猕猴桃叶片、叶片愈伤组织为材料 ,研究了影响原生质体分离和培养的因素 结果表明 ,原生质体分离 ,酶液配方 2优于配方 1,成龄叶片优于幼龄叶片 ;培养方法以浅层培养法最好 ;改良MS液体培养基培养愈伤组织原生质体 ,5～ 7d出现第 1次细胞分裂 ,14d出现第 2次细胞分裂 ,30d形成多细胞团 ,5 5d形成肉眼可见的小愈伤组织更多还原

【Abstract】 Leaves and leaf calli of Actinidia arguta were used to study the factors affecting protoplast isolation and culture.The results indicated that enzyme solution II was better than solution I and mature leaf better than young leaf for isolatong protoplasts.The shallow layer culture method is the best for culturing protoplasts.When protoplasts isolated from in vitro leave callus were cultured in a modified liquid MS medium(devoid of NH 4NO 3),they divided continuously.The first division of the protoplast cell was seen on 5～7 th ays of culture,the second one on 14 th ay.Regenerated cell colonies wrer seen on 30 th day of culture,and finally the colonies developed into calli visible to the naked eyes on 55 th day.更多还原