【摘要】 为了寻找在低温条件下降解含酚污水的微生物 ,利用低温条件 (6℃ )从自然界筛选出 2株在低温条件下有较强的生长率及代谢活性的菌株 ,经 30 0 μg/mL酚的诱导 ,分别培养于不同酚浓度及不同温度条件下 ,经过诱导的菌株耐酚和降解酚的能力有了显著的提高 .菌株XD 1的酚最大致死浓度由原来 30 0 μg/mL提高到 12 0 0μg/mL ;菌XX 2由 30 0 μg/mL提高到 10 0 0 μg/mL ,6℃下 6 0 0 μg/mL酚浓度的降解时间由原来的 7d缩至 4d .经生理生化反应鉴定 ,XD 1为Pseudomasstuzerisp ;XX 2为AcinetobactergroupNO Ⅰsp ,且其对苯酚的降解主要发生在生长对数期 .此菌株的研究可望在低温条件下对水体中酚污染的去除起到重要的作用 .更多还原

【Abstract】 Based from the view of treating with polluted water including phenol by microbiology under low temperature, this article explains under the low temperature of 6 degree centigrade how to screen two strains of bacteria with relatively powerful growth rate and metabolism vitality. Then, induced by 300*#μg/mL phenol, separately nurtured under the conditions of different phenol concentrations and temperatures, the anti-phenol and phenol degrading ability of the induced bacteria improve remarkably. The maximum fatal phenol concentration of the XD*.1 and XX*.2 bacteria increases separately from 300*#μg/mL to 1200*#μg/mL and 300*#μg/mL to 1000*#μg/mL. Under 6 degree centigrade, the degrading time for 600*#μg/mL phenol concentration decreases from 7 days to 4 days.By physical and biochemistry identification ,XD*.1 belongs to Pseudomas stuzeri sp.;XX*.2 belongs to Acinetobacter group NO-Ⅰsp.And we hope that our researching on these bacteria will play an important protective role to the environment under low temperatures.更多还原