【摘要】 采用高效毛细管电泳法成功地分离测定了甘草中甘草酸的含量 ,在 pH =5 .8的5 0mmol/L磷酸缓冲液 ,检测波长 2 5 2nm ,分离电压 2 5KV ,进样压力 2 0mm汞柱 ,温度 3 0℃的检测条件下 ,标准溶液在 0 .0 4 4 8mg/ml～ 0 .2 2 4mg/ml范围内与峰面积成良好的线性关系 ,回归方程为 :Y =4.1 6× 1 0 6x - 8.94× 1 0 4 ,相关系数r=0 .993 9,具有较高的灵敏度和准确度。该法操作简便、迅速、可靠、测试费用低更多还原

【Abstract】 This was the first time that High Performance Capillary Electrophoresis was used to determine the glycyrrihizin acid content in the plant Glycyrrhiza Uralensis. Ficsh. The capillary used was 75 μm i.d.and 62cm in length. UV detection was set at 252nm.The calibration curve showed good linearity. The recovery of glycyrrihizin acid ranged from 96%～99%.This method is simple, rapid and reliable.更多还原