【摘要】 目的 为探索提高库血内型肝炎病毒（ＨＣＶ）的检出率。方法 采用逆转录套式聚合酶链反应（ＲＴ－ＰＣＲ）技术，检测４ ５３０份抗－ＨＣＶ阴性的库血ＨＣＶ－ＲＮＡ。结果 ４ ５３０份库血中ＨＣＶ－ＲＮＡ阳性５５份，阳性率１．２１％，其中个体献血者血液２ １１０份，ＨＣＶ－ＲＮＡ阳性４０份，阳性率１．９０％；无偿献血者血液 ２ ４２０份，ＨＣＶ－ＲＮＡ阳性 １５份，阳性率０．６２％。结论ＲＴ－ＰＣＲ可用于检测库血ＨＣＶ－ＲＮＡ，以减少因输血造成ＨＣＶ感染。更多还原

【Abstract】 Objective To study on improvement of detection rate of hepatitis C virus in banked blood. Methods Nested reverse transcriptional-polymerase chain reaction (RT-PCR) was used to detect HCV-RNA in 4 530 shares of banked blood in which anti-HCV was negative. Results There were 55 shares of positive HCV-RNA in 4 530 with the positive rate of 1. 21%. Among them, there were 40 shares of positive HCV-RNA in 2 110 individual blood donors with the positive rate of 1. 90% ,and 15 shares of positive 1-IC V-RNA in 2 420 volunteering blood donors with the positive rate of 0. 62%. Conclusions RT-PCR can be used to detect HCV-RNA in banked blood to reduce HCV infection because of blood transfusion.更多还原