【摘要】 目的　 探讨 Ag NOR染色技术在淋巴结病变针吸细胞学涂片染色效果及对淋巴结病变定量诊断的价值。方法　 应用胶银染色法 ,对 2 0 8例淋巴结病变针吸细胞学涂片进行 Ag NOR染色 ,油镜下行 Ag NOR颗粒计数和形态、分布观察。结果　 Ag NOR颗粒均值在 81例淋巴结转移癌为( 8.84± 3.1 2 ) ;30例非霍奇金病 ( NHL)为 ( 9.42± 2 .78) ;1 1例霍奇金病 ( HL)为 ( 4 .69± 1 .0 8) ;34例慢性淋巴结炎为 ( 1 .32± 0 .2 8) ;2 8例淋巴结结核为 ( 1 .46± 0 .34) ;2 4例增生性淋巴结炎为 ( 2 .2 6± 0 .45 )。良恶性淋巴结病变细胞 Ag NOR颗粒数之间存在高度显著性差异 ( P <0 .0 1 ) ,并且二者之间的 Ag NOR颗粒大小及分布亦呈现明显的不同。结论　 Ag NOR染色技术是定量鉴别良恶淋巴结病变 ,尤其是 NHL与增生性淋巴结炎的良好方法更多还原

【Abstract】 Objective To explore the effect of AgNOR dyeing for the lymph node smears by fine needle aspiration cytology(FNAC) and the value of AgNOR dyeing in quantitative diagnosis of lymphadenopathy.Methods The lymph node smears of 208 cases with lymphadenopathy were stained with AgNOR dyeing,the numbers of AgNOR granule counted and their shape and distribution observed under oil immersion lens.Results The average value of the numbers of AgNOR granule were 8.84±3.12 in 81 cases of lymph node metastatic carcinoma;9.42±2.78in 30 cases of NHL disease ,4.69±1.08 in 11 cases of HL disease,1.32±0.28 in 34 cases of chronic lymphadenitis,1.46±0.34in 28 cases of tuberculous lymphadenitis;and 2.26±0.45 in 24 cases of hyperplasia of lymph node.There was a remarkable difference in the numbers of AgNOR granule between the malignant and benign cells lymphadenopathy(P<0.001).The shape and distribution of AgNOR granule were also obviously different.Conclusion The technology of AgNOR dyeing was a good method in quantitative differentiation of both malignant and benign lymphadenopathy,especially of NHL and hyperplasia of the lymph nodes.更多还原