【摘要】 目的 :观察 7d龄大鼠缺氧缺血性脑病 (HIE)模型中脑细胞凋亡的规律 ,为寻求最佳治疗时间提供一定的动物实验依据。方法 :利用 7d龄SD大鼠建立HIE动物模型 ,用流式细胞仪测定缺氧缺血 (HI)后 2h～ 7d内不同时间脑细胞凋亡率。结果 :7d龄正常新生大鼠HI后 48h细胞凋亡达高峰。结论 :早期用药 (2 4h～ 48h内 )将是抑制细胞凋亡 ,提高疗效的关键更多还原

【Abstract】 Objective: To explore the pattern of neuronal cells apoptosis due to hypoxic ischemia (HI) in newborn rats and provide experimental data for clinical treatment of the lesion. Methods: The model of hypoxic ischemic encephalopathy (HIE) was established in normal newborn rats of 7 days old. The percentage of neuronal apoptosis in the sample was measured with the flow cytometry during 2 hours to 7 days after HI. Results: After 48 hours of HI, the number of apoptotic cells was up to the peak level. Conclusion: The best time for treatment might be during 24 48 hours after HI, which prohibits the apoptosis and protects brain from HI damage.更多还原