【摘要】 以鸡蛋清为原料 ,控制温度 5℃ ,采用 72 4型树脂吸附 ;再用 φ(H2 SO4 ) =1%的稀硫酸分四次洗脱 ,用NaOH调pH =8 5～ 9 0 ,除去杂质 ;再用c(HCl) =3 0mol/L的盐酸调pH =3 5 ,加入w (NaCl) =5 %的溶液使溶菌酶析出 ,用无水丙酮 0℃干燥即得溶菌酶成品。更多还原

【Abstract】 Using egg white as raw material and 724 resin as adsorbent,eluting 4 times with 1%(V/V) H 2SO 4,regulating pH to 8 5～9 0 with NaOH and removing foreign substance,then regulating pH to 3 5 with 3 0 mol/L HCl and adding 5%NaCl,lysozyme is precipitated and refined in acetone at 0 ℃.更多还原