【摘要】 目的　筛选和克隆卵巢上皮癌相关基因。　方法　用 3种锚定引物和 8种随机引物 ,共 2 4种组合进行PCR扩增 ,通过 DD- RTPCR分析正常卵巢上皮和卵巢上皮癌 m RNA的基因表达差异 ,将差异片段分离出来 ,并回收 ,克隆和进行斑点杂交鉴定、DNA序列测定 ,并通过国际互联网络检索 Gen Bank进行同源性分析。　结果　获得30个差异表达的片段 ,经斑点杂交初步筛选 ,克隆 11个差异显示的阳性片段。选取其中的 5个进行序列分析。　结论　计算机同源性分析表明 ,5个均为新基因片段 ,已收录入 Gen Bank。更多还原

【Abstract】 Objective To identify genes related to the development of ovarian cancer by studying the differential expression genes between normal ovarian epithelia and ovary epithelia cancer. Methods The RNA of both the normal ovary epithelia and ovarian cancer cell line(3AO,SKOV3) was isolated for differentially expressed genes using differential display RT-PCR(DD-RTPCR).Three sets of anchor primers and 8 sets of arbitrary primers,total 24 primer combinations were used for DD-RTPCR amplification.These differentially expressed fragments were recovered,cloned,further analyzed by dot blot,and then sequenced these clones and compared with GenBank/EMBL databases. Results After 30 differential display bands preliminarily screened by dot blot hybridization,11 differential display fragments were cloned and 5 differential expressed fragments were sequenced. Conclusion Sequencing and homology analysis indicated that these cDNA-fragments did not match any genes published previously,they are novel gene sequences,which have been submitted to GenBank.;更多还原