【摘要】 为探讨胶质细胞源性神经营养因子 (GDNF)对大鼠脊髓运动神经元的作用 ,采用免疫组织化学方法 ,观察GDNF在大鼠生后 1、3、7、10、14和 2 8天腰段脊髓中的分布。结果发现 ,在生后 1、7和 2 8天脊髓灰质前角运动神经元有强阳性染色 ,生后 3天脊髓灰质前角运动神经元呈弱阳性染色 ,生后 1、3、7和 2 8天脊髓灰质后角神经元和少量胶质细胞也呈阳性反应。此外 ,在各组大鼠腰段脊髓后根内的胶质细胞亦可见GDNF免疫阳性反应。提示GDNF可能对脊髓运动神经元的发育、存活以及功能的正常发挥起重要作用。更多还原

【Abstract】 In order to explore the effect of glial cell line derived neurotrophic factor (GDNF) on the motor neurons of the spinal cord, the present study investigated the localization of GDNF in the lumbar segment of the spinal cord on postnatal 1、3、7、10、14 and 28 days in rats with immunohistochemical staining method. The results showed that the positive GDNF immunoreactivity was present in the motor neurons of the ventral horn on postnatal 1、7 and 28 days, the motor neurons on postnatal 3 days were also weakly stained in rats,and some neurons of the dorsal horn and some glial cells had positive immunoreactivity. Furthermore, GDNF was found in the glial cell of the dorsal root in all groups of the postnatal spinal cord. The present results suggested that GDNF might play important roles in the development and function of the motor neurons of the spinal cord. More experiments should be done to explain why there is no positive immunoreactivity in the motor neurons between postnatal 7 and 28 days.更多还原