【摘要】 观察不同浓度的褪黑激素 (Melatonin ,MLT)对原代培养的大鼠垂体催乳素瘤细胞增殖的影响。用 80～10 0g体重的雄性Sprague Dawley(SD)大鼠 ,皮下埋置雌二醇诱致垂体催乳素瘤 (prolactinoma)形成后 ,取出瘤体 ,经消化、过滤、离心 ,用SFFD培养基重悬细胞 ,得细胞悬液 ,浓度为 0 75× 10 6/mL ,转入 2 4孔培养板中并加入相应剂量的MLT使其终浓度 (mol/L)分别为 10 -5、10 -7、10 -9、10 -11和 10 -13 ,另设空白对照组 ,于 37℃、5 %CO2 条件下培养 1h ,再在每孔中加入 2 μCi [3H] TdR继续孵育 2 4h后测counts/min值。结果显示 ,在对照组和各剂量 (mol/L)MLT用药组 10 -5,10 -7,10 -9,10 -11和 10 -13 的 [3H]-TdR掺入率分别为 2 0 38 75± 186 84,142 1 75±6 4 49,12 38 2 5± 6 1 72 ,92 4 0 0± 6 4 44 ,10 33 2 5± 12 7 2 2和 110 3 2 5± 15 1 5 3counts/min。表明 10 -5～10 -13mol/L的MLT能有效抑制 [3H] TdR在原代培养的垂体催乳素瘤细胞中的掺入 ,P <0 0 0 1。其中 ,以 10 -9mol/L的MLT的抑制作用最强 ,达 5 4 6 8%。提示在培养条件下 ,10 -5～ 10 -13 mol/LMLT能有效地抑制E2 诱导的垂体催乳素瘤细胞的增殖。更多还原

【Abstract】 In present study, we have examined the effect of melatonin at different concentrations on the proliferation of 17 β estrogen(E2) induced prolactinoma cells of rats The prolactinomas were established by implanting E 2 laden silastic capsule subcutaneously in 80～100 g weight Sprague Dawley (SD) male rats Ninety days after E 2 capsules implantation, the animals were killed, and prolactinomas were removed The suspension of tumor cells was obtained by enzymatic digestion, filtration, centrifugation, and resuspension in incubating medium 1 ml aliquot of the cell suspension containing 0 75×10 6 cells were distributed into each well of 24 well cell culture plate respectively Then MLT was added to appropriate wells to make the final concentration 10 -5 to 10 -13 mol/L Respective quantity of ethanol was added to control wells Then the cells were incubated at 37℃ in 5 % CO 2 After 1 h, 74KBq(2μCi) / 2 μL of [ 3H] TdR was added in 50 μL of the medium The incubation was terminated 24 h later, and then the radioactivity was counted The result showed that the radioactivity (counts/min) of [ 3H] TdR in tumor cells in control group and test group at doses of 10 -5 , 10 -7 , 10 -9 , 10 -11 and 10 -13 mol/L melatonin were 2038 75 ±186 84,1421 75 ±64 49, 1238 25 ± 61 72, 924 ± 64 44, 1033 25± 127 22 and 1103 25 ±151 53, respectively The results suggested that Melatonin in the concentration range 10 -5 ～10 -13 mol/L could inhibit the proliferation of 17 β estrogen induced rat prolactinoma cells更多还原