【摘要】 以质粒pBI12 1作外源DNA ,对基因枪介导转化水稻花药愈伤的有关参数进行了优化 ,优化值分别为射程 9cm ,枪膛氦气压力 110 0psi,真空度 36 0 0～ 3733Pa ,轰击次数 2次。轰击前后愈伤经 0 .4mol/L甘露醇处理可提高转化效率。在优化条件下将Xa2 1基因导入籼型光敏核不育水稻W945 1S ,获得了单倍体植株 ,经人工染色体加倍得倒二倍体转基因植株。白叶枯病菌接种鉴定结果表明其抗性有明显提高更多还原

【Abstract】 The transformation condition of rice anther derived callus by biolistic bombardment was investigated with plasmid pBI121 as donor DNA. The results showed that the optimums of target distance, helium gas pressure, vacuum and the times of bombardment were 9 cm, 1?100～1?300 psi, 3?700～3?833 Pa and 2 respectively. The osmotic treatment of callus with 0.4 M mannitol for 4 h before and 16 h after bombardment had a good influence on transformation efficiency. Xa 21 gene was introduced into rice W9451S on the optimum condition, and the diploid transgenic rice plants were obtained through chromosome doubling of the haploid plants. The transgenic plants showed resistance to bacterial blight.更多还原