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MTT法测定矿物质提取物MICOM及中药提取物AN4的体外抗肿瘤活性

Detecting the anticancer effects of mineral-extract-MICOM and TCD-extract-ANT4 by MTT technique in vitro

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【作者】 宋维华许超迁孙建平杨宝峰何树庄

【Author】 SONG Wei hua,XU Chao qian,SUN Jian ping, et al (Department of Pharmacology, Harbin Medical University,Harbin 150086,China)

【机构】 哈尔滨医科大学药理学教研室哈尔滨医科大学药理学教研室 黑龙江哈尔滨150086黑龙江哈尔滨150086黑龙江哈尔滨150086

【摘要】 目的 用体外培养法研究矿物质提取物MICOM及中药提取物AN4对肝脏肿瘤细胞生长的抑制作用。方法 采用MTT法进行研究。结果 细胞在MICOM、AN4处理 72h后 ,测得MICOM的IC50 为 9.32 μg/ml。结论 矿物质提取物MICOM在体外可有效抑制HLE细胞的生长 ,而AN4在体外抑制细胞生长作用不明显

【Abstract】 Objective To study the anticancer effects of MICOM (mineral extract) and TCD(traditional Chinese drug) extract AN4 on growth inhibition in HLE tumor cells. Methods HLE cells were treated with MICOM and AN4 at different concentration. The effects of MICOM and AN4 were observed by MTT assay. Results After HLE cells were treated by MICOM and AN4, the growth of tumor cells were inhibited in vitro . IC 50 of MICOM was 9.32μg/ml after HLE cells were treated with MICOM for 72h.Conclusion MICOM can inhibit the proliferation of HLE cell significantly, while the effect of AN4 is not obvious.

【关键词】 抗肿瘤药MTT法HLE细胞细胞培养
【Key words】 antineoplastic agentsMTT assayHLE cellcell culture
  • 【文献出处】 哈尔滨医科大学学报 ,Journal of Harbin Medical University , 编辑部邮箱 ,2001年06期
  • 【分类号】R735.7
  • 【被引频次】15
  • 【下载频次】261
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