【摘要】 目的　提高培养大脑皮质神经元的产率以及活性 ,使实验的细胞模型有良好的一致性和可比性。方法　实验分析了大脑皮质神经元培养过程中 ,从选材、取材到分散细胞等一系列步骤中维持细胞活性的内在因素 ,应用细胞形态学指标 ,Trypanblue染色计数分析以及染色观察。结果　每侧大脑皮质得到细胞约 2 .5× 10 6～ 3.0× 10 6个 ,而且种植不同时间 6 ,12 ,48h细胞存活率均在 95 %以上。结论　该法是较好的大脑皮质神经元培养方法。更多还原

【Abstract】 Objective To study increasing the yield and viability of cells from the brain cortical layer in vitro , so that obtain a cell model that has good uniformity and comparability.Methods We analysed the internal factors that maintance cell viability during culturing cells of the brain cortical layer.The morphology and the number of viable cells were used as indexes to evaluate the improved experiment process.Results 2.5×10 6～3.0×10 6 cells could be obtained from each hemisphere with the new method,and the viability of cells reached above 95% at different point of time after culture.Conclusion This new method is a good alternative for increasing the yield and viability of neural cells in culture.更多还原