【摘要】 目的　探讨白细胞介素、干扰素 α2 a、bcr- abl反义寡核苷酸对慢性粒细胞白血病骨髓体外净化效果。方法　采用体外克隆形成培养技术 ,分别用白细胞介素、干扰素 α2 a、bcr- abl反义寡核苷酸不同的组合方案 ,体外净化 5例慢性粒细胞白血病 (CML)骨髓。结果　联用 IL- 2、IFN- α2 a(各 80 0 u/ m l)、bcr- abl AS- ODN(30 μg/ ml)方案 ,对白血病细胞克隆形成单位 (L- CFU)抑制作用有显著差异 (P<0 .0 1) ,而相同条件下 ,GM- CFU及 L- CFU的集落存活率分别为 2 6 .91%和 3.49% (P<0 .0 1)。结论　联用 IL- 2、IFN- α2 a(各 80 0 u/ m l)、bcr- abl AS- ODN(30 μg/ml)方案 ,选择性体外净化慢性粒细胞白血病细胞的效果好 ,可用于临床净化 CML 骨髓更多还原

【Abstract】 Objective To investigate the effect of IL 2, IFN and bcr abl AS ODN on bone marrow purging in vitro for patients with chronic myelogenous leukemia (CML). Methods By amplifying in vitro clone forming culture, and purging the bone marrow of 5 CML patients through different combined use of IL 2, IFN and bcr abl AS ODN, we compared the effects of 4 combined regimens.Results It was found that the suppressive effects of the 4th regimen combining IL 2 (800U/ml), IFN (800u/ml) and bcr abl AS ODN (30μg/ml) on lenkemia cell clone forming unit (CFU) were significantly different from the suppressive effects of the other 3 regimens ( P <0 01). With the use of the 4th regimen, the GM CFU and L CFU survival rates were 26.91% and 3.49% respectively ( P <0.01).Conclusiont The combined regimen of IL 2 (800U/ml), IFN (800u/ml) and bcr abl AS ODN (30μg/ml) has good effect on selective CML bone marrow purging in vitro and can be used in clinical purging of CML bone marrow.更多还原