【摘要】 目的 :了解口腔癌的颈淋巴结转移机制 ,观察具有介导淋巴管内皮细胞和淋巴管增殖的血管内皮生长因子 C(VEGF C)在口腔癌中的表达。方法 :采用免疫组化染色 ,光镜及图像分析观察并确定VEGF C在正常口腔粘膜、白斑及鳞癌等组织中的表达。结果 :在正常及病变上皮组织中皆有VEGF C的表达 ,部分淋巴管也有表达。但鳞癌组织表达明显高于正常口腔粘膜及白斑 ;鳞癌表达强度与病理分级、淋巴结转移密切相关 ,与临床分期无关。结论 :VEGF C通过介导淋巴管内皮细胞及淋巴管增殖有利于颈淋巴道转移的发生 ,VEGF C检测可作为预测淋巴道转移及预后判断的指标之一。更多还原

【Abstract】 Objective: Vascular endothelial growth factor C (VEGF C) is known to be capable of inducing cell proliferation of lymphatic endothelia and development of lymphatic vessels. Thus, in the current study the expression of VEGF C was investigated to understand the mechanism of lymphatic metastasis of oral cancer. Methods:VEGF C protein expression in benign and malignant oral lesions was investigated with an immunohistochemical staining assay, followed by light microscopic examination and image analysis. Results: Immunohistostaining of VEGF C was observed in normal, benign and malignant epithelia, as well as in some lymphatic vessels. The VEGF C positive rate was significantly higher in squamous carcinomas than in normal tissues and benign lesions. It was also noted that in squamous carcinomas the expression level of VEGF C correlated well with pathological grades and stages of lymph node metastasis, but not with clinical grades. Conclusion: These findings suggest that VEGF C may promote lymphatic metastasis of oral cancer, and it is possible to be used as a predictor for the lymphatic metastasis and prognosis of oral cancer. \;更多还原