【摘要】 采用正交实验法分析出黑曲霉 3.316纤维素酶系各组分最适酶解条件 ,其组合分别为 C1酶 (p H5.0 ,4 5℃ ) ,Cx 酶 (p H3.5,6 5℃ )和 βG酶 (p H4 .5,6 5℃ ) .在液体发酵条件下 ,添加质量分数为 0 .0 0 3的碳酸钙时 ,各组分酶活性最高 ,其中以 C1酶明显增高 ,Cx 酶几乎无影响 ,βG酶则明显降低 .C1和 Cx 比酶活只有在碳酸钙质量分数小于 0 .0 0 5时才有提高 ,βG酶则明显降低 .添加蛋白胨 ,C1和 βG酶的最高酶活在碳酸钙质量分数小于 0 .0 0 3时有增加 ,Cx 酶则无明显变化 .碳酸钙和蛋白胨对各组分最高酶活形成时间均无影响 .在各组分酶分泌规律方面 ,仅有碳酸钙对 C1和βG酶有一定影响更多还原

【Abstract】 By adopting orthogonal test, the optimel enzymolyis condition for respective component of cellase system from Aspergillus Niger 3.316 induced liquid fermentation are found to be pH5.0, 45 ℃ for enzyme C 1, and pH3.5, 65 ℃ for enzyme C x and pH4.5, 65 ℃ for enzyme β G. Under the condition of liquid state fermentation, the addition of CaCO 3 in a concentration of 0.3% leads to the highest enzyme activity of varions components. In which that of C 1 obviously increases, that of C x is almost unaffected, while that of β G obviously decreases. The enzyme activity ratio of C 1 and C x increases only if CaCO 3 keeps in a concentration below 0.5%, while C x shows no significant change. The addition of peptone leads to the highest enzyme activity of C 1 and β G of CaCO 3 keeps in a concentration below 0.3% while it leads to insignibicant change of C x . The formation time of highest enzyme activity of respective lomponent are uneffected by CaCO 3 and peptone. Regarding to the rule of enzyme secretion of respective component, only CaCO 3 shows a certain effect on C 1 and β G.更多还原