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呼吸道合胞病毒地方株SH蛋白基因序列分析

Molecular analysis of SH gene of local respiratory syncytial virus strains isolated in China

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【作者】 王予芳王之梁钱渊耿学辉万里涛邓洁刘成贵常汝虚刘常清朱宗涵

【Author】 WANG Yufang 1) ,WANG Zhiliang 2) ,QIAN Yuan 1) ,GENG Xuehui 1) ,WAN Litao 1) ,DENG Jie 1) ,LIU Chenggui 1) ,CHANG Ruxu 2) ,LIU Changqing 3) ,ZHU Zonghan 1) 1)Capital Institute of Pediatrics, Beijing Municipal Labo

【机构】 首都儿科研究所北京市感染与免疫中心实验室!北京100020长春市儿童医院!长春130000

【摘要】 目的 :分析我国呼吸道合胞病毒 (RSV)地方株SH蛋白基因的遗传特点、变异特征。方法 :从本室保存的不同年份、不同地区、不同流行特征的呼吸道合胞病毒地方株中抽取 7株 ,分别用RT PCR扩增其SH蛋白基因并克隆到PTZ18R载体中进行了序列测定和分析。结果 :我国RSV地方株间SH蛋白基因相比较变异的部位及形式很相似 ,地方株间核苷酸全序列的同源性为 97.0 %~ 10 0 %。结论 :RSV在我国不同地区的不同流行特征与SH蛋白基因的变异无明显关系

【Abstract】 Aim: To study the molecular structure of SH gene of local respiratory syncytial virus strains .Methods: The SH genes of 7 local respiratory syncytial virus(RSV) strains, which were isolated from children with varied epidemiological characteristics in different areas and different periods,were amplified by PT PCR and then cloned and sequenced.Results:The variations in locus and pattern among the local strains were similar and the homology of nucleotide sequences was 97 0%~100% Conclusion:The data indicate that the variation of the SH gene is not related to the different epidemiological characteristics in China.

  • 【文献出处】 河南医科大学学报 ,Journa of Henan Medical University , 编辑部邮箱 ,2001年05期
  • 【分类号】R373.1
  • 【下载频次】56
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