【摘要】 目的 :探讨胃癌组织p16基因纯合缺失与突变的发生率及其临床意义。方法 :PCR方法扩增p16基因外显子 1(E1)及外显子 2 (E2 ) ,检测等位基因纯合子缺失 ;应用单链构象多态性 (SSCP)方法分析基因突变情况。结果 :2 0例胃癌组织中E1、E2纯合性缺失分别为 4例 (2 0 % )及 2例 (10 % ) ;SSCP未检出E1突变 ,E2有 2例出现泳动易位的异常单链 ,其中 1例 (Ⅲa期 ,低分化腺癌 )癌与癌旁组织均出现异常 ,2例异常标本均为进展期 ,LOH(+)胃癌。结论 :p16基因异常是胃癌发生发展中一重要事件 ,突变多见于外显子 2 ,可能与癌肿进展有关更多还原

【Abstract】 Aim:To investigate the frequencies of homozygous deletion and mutation of p16 gene and to evaluate the clinical significance.Methods:PCR technique was used to detect homozygous deletions of exon 1 and exon 2 of p16 gene and single strand conformation polymorphism (SSCP) technique was used to detect the genic mutation.Results:The homozygous deletion ratio of exon 1 and exon 2 of p16 gene in 20 gastric cancer tissues was 4 out of 20 cases and 2 out of 20 cases, respectively. In SSCP no mutation was found in exon 1 of p16 gene, while abnormal single strands were found in exon 2 in 2 cases, one of which included gastric cancer tissue and adjacent cancer tissue (belonged to Ⅲa stage, poorly differentiated cancer). The positive tissues were both advanced gastric cancers and LOH(+) cases.Conclusion: The abnormality of p16 gene is an important event in the progression of gastric cancer. The mutation focuses on exon 2, and may be associated with the development of gastric cancer.更多还原