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PAC克隆DNA制备方法的改进
Improvements on the DNA Preparation Methods of PAC Clones
【摘要】 为了解决PAC克隆DNA制备中存在的若干问题,我们探索了几种改进方法,使提取的PAC克隆DNA质量明显改进,能较好地满足我们对儿童急性淋巴细胞白血病6q21缺失区抑癌基因克隆的研究目的.
【Abstract】 To solve problems occurring in the DNA preparation of the PAC clones, we used modified methods, which made obvious improvement on the quality of the PAC clone DNA. The DNA extracted may meet the requirement of our research on cloning of a tumor suppressor gene located in the minimum deletion region in 6q21 associated with child acute lymphatic Leukemia.
【基金】 湖南省特聘教授基金项目(25000613)
- 【文献出处】 湖南师范大学自然科学学报 ,Journal of Naturnal Science of Hunan Normal University , 编辑部邮箱 ,2001年02期
- 【分类号】Q785
- 【下载频次】68