【摘要】 以种子萌发无菌苗研究台湾相思的组织培养,结果发现子叶、下胚轴是诱导愈伤组织的理想外植体,最适培养基为MS+BA2+2,4-D0.2+3％蔗糖.子叶及下胚轴诱导的愈伤组织在MS+BA+NAA0.2-0.4培养基中的出芽效果较好,子叶直接在MS+4-PU0.5培养基中诱导丛生芽效果最好,出芽率达90％.根诱导培养基为MS+IBA2+NAA1,生根率为27.8％.更多还原

【Abstract】 Tissue culture of Acacia confusa with the materials from the sterile seedlings as explants was studied. The results showed that cotyledon and hypocotyl were successful for callus induction, and that the optimum culture condition was MS basal medium supplemented with 2 mg/L BA, 0.2 mg/L 2,4- I) and 3% sucrose. The suitable medium for differentiation of buds irom the calli of cotyledon and hypoeotyl was MS medium added 4 mg/L BA and 0.2-0.4 mg/L NAA. Cotyledon cultured in the medium of MS + 4 - PU0.5 was the best for bud formation.The frequency of bud induction was 90% .The medium of MS + IBA2 + NAA1 was effective to induce root. Rooting percentage added up to 27.8 % .更多还原