【摘要】 提出了测定微量蛋白质的新方法。蛋白质,包括牛血清白蛋白(BSA)人血清白蛋白(HSA),牛血红蛋白(HB)能在PH4.3的酸性介质中与桑色素结合,使桑色素的光散射信号提高。在最佳实验条件下,BSA浓度在0.5—8.0μg/ml范围内呈线性关系。将这一方法用于合成样品中微量蛋白质的测定,结果满意。更多还原

【Abstract】 A determination method of proteins is proposed by using a common spectrofluorometer to detect intensity of the scattering light. proteins, including bovin serum albumin(BSA) , human serum albumin (HSA) , hemoglobin (Hb),can combine with morin in the pH4.3 and produce an enhanced light scattering signal.In optimization conditions,the linear range is 0.5-8.0 ug·ml-1 for BSA.The method was applied to determine proteins in synthetic samples with satisfactory results.更多还原