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甘肃滩羊血清酯酶和α-淀粉酶多态性分析

The Polymorphism of Gansu Tan Sheep on Es andα-Amy loci in Gansu province

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【作者】 李积友李秉诚刘霞折富有武广善宋祖才田世雄赵承银

【Author】 LI Ji-you1,LI Bin-cheng1,LIU Xia2,ZHE Fu-you5,WU Guang-shan1, SONG Zu-cai3,TIAN Shi-xiong4, ZHAO Cheng-yin5 (1. Gansu Livestock Production Technique Central Station; 2. College of Animal Medicine, Gansu Agricultural University, Lanzhou 730070, China; 3. L

【机构】 甘肃省畜牧技术推广总站甘肃农业大学动物医学院景泰县畜牧工作站皋兰县畜牧工作站靖远县畜牧工作站景泰县畜牧工作站 兰州730030兰州730030兰州730070甘肃景泰730400甘肃皋兰730200甘肃靖远7

【摘要】 运用聚丙烯酰胺凝胶电泳技术(PAGE)对甘肃滩羊产区皋兰、景泰及靖远3293只滩羊的血清酯酶(Es)、α-淀粉酶(α-Amy)基因座多态性进行了检测分析。结果表明:甘肃滩羊Es基因座存在3种基因型Es++ 、 Es+- 、 Es-- ,它们由Es+、Es-两个共显性等位基因控制,其中Es+-基因型在3个群体中均占优势,频率分别为0.620 0(皋兰)、0.666 6(景泰)、0.730 0(靖远);在皋兰群体和景泰群体中,Es-基因占优势,基因频率分别为0.540 0和0.548 4,而在靖远群体中优势基因则为Es+,其基因频率为0.525 0。在α-Amy基因座上没有检测到多态性。

【Abstract】 Polyacrylamide gel electrophoresis(PAGE) was applied to study polymorphism of Es and α-Amy loci of 293 Tan sheep in Gaolan county, Jingtai county and Jingyuan county of Gansu provine. The results showed that the locus of Es appeared 3 genetypes Es++, Es+-, Es which was decided by Es+ and Es- genes. Among 3 genetypes,Es+- was superior in all populations, the genetype frequencies were 0.6200(Gaolan),0.6666(Jingtai) and 0.7300(Jingyuan) resp ectively. Es- was dominant genes in Gaolan population and Jingtai population whose frequencies were 0.5400 and 0.5484 respectively correlationship with 0.5250 Es+ in Jingyuan population which was relatively superior. Monomorphism was investigated on α-Amy locus.

【关键词】 滩羊血清酯酶α-淀粉酶多态性分析
【Key words】 Tan sheepEsα-Amypolymorphism
  • 【文献出处】 甘肃农业大学学报 ,Journal of Gansu Agricultural University , 编辑部邮箱 ,2001年04期
  • 【分类号】S826.1
  • 【被引频次】14
  • 【下载频次】60
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