【摘要】 为了从细胞凋亡角度探讨鬼臼乙叉甙 (4′- demethylepipodophyllotoxin- 9-(4 ,6- O- ethylidene- β- D- glucopyranoside)抗肿瘤作用的机理。用活细胞计数法、克隆形成法及 MTT比色法观察了其对 NB4细胞的生长抑制作用 ;用光学显微镜及电子显微镜观察了凋亡细胞的形态学变化 ;用琼脂糖凝胶电泳观察了 DNA的梯形改变。结果发现 :鬼臼乙叉甙能显著抑制 NB4细胞的生长增殖及诱导其凋亡。诱导凋亡是鬼臼乙叉甙抗肿瘤作用的机理之一更多还原

【Abstract】 To study the antitumor mechanism of etoposide(4′ demethylepipodophyllotoxin 9 (4,6 O ethylidene β D glucopyranoside), the growth inhibition effects of etopside on NB4 cell line were studied by survival cell count, colony formation assay and MTT colorimetry. Apoptotic morphology was observed under light and electron microscopes. DNA ladder was observed using agarose gel electrophoresis. The results showed that etoposide could markedly inhibit NB4 cell growth and proliferation and significantly induce NB4 cell apoptosis. This study suggests that induction of apoptosis is one of the antitumor mechanisms of etoposide.更多还原