【摘要】 用免疫细胞化学和原位杂交技术探讨 G、 D细胞及胃泌素 m RNA与肠化生的关系。标本来自胃镜活检的胃粘膜。结果显示 ,在与大肠化生区相邻的胃粘膜 ,G细胞突然消失 ,假幽门腺化生也缺乏 G细胞 ,而小肠化生仍保留少数 G细胞 ;D细胞不仅见于小肠化生 ,而且也出现在假幽门腺化生以及某些大肠化生区。胃泌素 m RNA仅限于 G细胞分布区 ,未出现在大肠化生区和假幽门腺化生区。 G细胞及胃泌素 m RNA在大肠化生区的消失 ,可能由于局部杯状细胞分泌的硫酸粘蛋白改变了局部的微环境 ,从而影响了 G细胞的分化与发育。至于假幽门腺化生区 G细胞及胃泌素 m RNA消失的原因还不清楚 ,应继续研究更多还原

【Abstract】 Using immunohistochemistry and in situ hybridization, we investigated the relationship between G, D cells, gastrin mRNA and intestinal metaplasia in human gastric mucosa. All specimens were obtained by biopsy during gastroscopy. The results, showed that G cells well absent both in mucosa adjacent to clonic metaplasia and pseudopyloric metaplasia while they were still present, though fewer than normal, in small intestinal metaplasia. D cells well found in pseudopyloric and small intestinal metaplasia and in some areas of clonic metalasia. Gastrin mRNA was only expressed in the G cell distributed region, The absence of G cells and its mRNA in clonic metaplasia may be associated with sulfomucin secreted by local goblet cells, which changes the local microenvironment and thus influences G cell differentiation and development. However, the reason of the absence of G cells and its Gastrin mRNA in pseudopyloric metaplasia is unclear.更多还原