【摘要】 应用免疫组织化学方法观察不同浓度蓖麻毒素作用于肝癌细胞不同时间后 ,对 i NOS的诱导作用。结果显示 ,未受蓖麻毒素作用的肝癌细胞胞浆 i NOS呈阴性反应 ;当受到蓖麻毒素诱导后 ,才能在胞浆内合成。不同浓度的蓖麻毒素对肝癌细胞诱导 i NOS的产生没有明显差别。而蓖麻毒素诱导 i NOS的表达有明显的时间依赖性 ,2 h没有表达 ,4h才出现 ,随着时间的延长 ,到 8h时仍然有明显的表达。由于 i NOS的合成涉及基因转录、蛋白质合成等过程 ,故需诱导数小时后显示酶活性 ,但一经诱导生成 ,酶活性持续时间长。蓖麻毒素诱导 i NOS的产生在抗癌应用中具有重要的价值更多还原

【Abstract】 To study iNOS production in hepatoma stimulated by ricin at different concentration and at different time. The results demonstrated, iNOS is negative in cytoplasm when hepatoma was not stimulated by ricin. After stimulating hepatoma with ricin, iNOS is positive in cytoplasm. The iNOS productin has not obviously difference when stimulating hepatoma by ricin at different concentration. However, iNOS express of ricin induction has distinctly tome dependent from 2h to 8h. The iNOS activity has persisted for a number of hours in hepatome cells. Because iNOS synthesis has related to gene transcription, protein synthesis and etc. The iNOS production of ricin induction may contribute to the clinical application. Our results indicate that ricin has great potential for the treatment of hepatoma cells.更多还原