【摘要】 以对甲酚为底物 ,利用从马铃薯中提取的多酚氧化酶的粗酶液 ,用多孔玻璃包衣琼脂疏水吸附法将多酚氧化酶固定化 ,并利用此固定化酶 ,考察了在非水介质中羟基化反应的最适宜条件 .在此条件下催化合成 4-甲基邻苯二酚的产率为 5 5 .8%更多还原

【Abstract】 Besides in nonaqueous enzymology, polyphenoloxidase can also be used in analytical chemistry as the enzyme electrode to identify phenols and cyanide, etc. The polyphenoloxidase inhibitors are widely used in industries of cosmotics and food. Polyphenoloxidase can change L \|tyrosin into L \|DoPA, a routine medicine for Pakinson’s disease. Tyrosonase has the character of ortho\|hydroxylation on benzene ring, so it has great significance in organic synthesis. Owing to its importance in medicine, the catalytic conversions of p \|cresol to 4\|methyl catechol and catechols to 4\|methyl\|1,2\|benzoquinone were intensively studied. In this work, we used a chemical method to reduce 4\|methyl\|1,2\|benzoquinone to 4\|methyl catechol, and to optimize the reaction conditions of catechol synthesis catalyzed by immobilized enzyme. The yield of 4\| methyl catechol catalyzed by immobilized enzyme is 55.8%.更多还原