【摘要】 小鼠经60 Coγ射线 6 .5 - 7.5Gy照射后 ,分别于肌袋内植入纯化牛BMP ,或腹腔内注入可溶性rhBMP - 2m ,或移植Pbk -hBMP - 2 /NIH3T3入腹腔内 ,观察动物的 30d活存情况 ,并检测几种造血参数。结果表明 ,pbBMP(纯化牛骨形成蛋白 )、hBMP - 2m (重组骨形成蛋白 - 2多肽 )Pbk -BMP - 2 /NIH3T3cellline(PBKhBMP - 2转基因细胞株 )对小鼠 6 .5、7.0、7.5Gyγ射线照射后急性放射造血损伤有治疗作用 :GM -CFU集落形成率、各项造血参数和 30d活存率均较对照组有显著性差异 (p <0 .0 1)。本工作结果提示 ,BMP对成年动物放射所致造血损伤具有治疗作用 ,其机理与促进已损伤的造血功能的修复作用有关。更多还原

【Abstract】 To investigate the effect of bone morphogenetic proteins (BMPs) on the acute hematopoietic sickness induced in mice. BMP, rhBMP-2m and PBK/ hBMP-2 -NIH3T3 cells were prepared as previously discribed . In this study, the effect of BMPs on hematopoiesis included some hematological parameters. The survival ratio on the 30th d post irradiation and formation of bone marrow CFU-GM colomies were detected. The experiments indicate that pbBMP (purified bovine bone morphogenetic protein) could increase the formation of bone marrow CFU-GM colomies ( p <0.05 ) at 10 th d after irradiation. rhBMP-2m increased the survival rate of mice irradiated by 7.5Gy. All mice in control group died in 30days, while 10%,15% and 35% mice survived when they were injected ip with 0.5mg, 1.0mg and 2.0mg of rhBMP-2m respectively. All hematological parameters of treated mice were significantly higher than those of control group ( p <0.01). PBK/ hBMP-2 -NIH3T3 cells were established and transplanted into mice irradiated by 7.0Gy γ ray by i.p. The survival r atio of treated mice higher than that of negative control group ( p <0.01), and all hematopoietic parameters were increased statistically significantly ( p <0.01). These data supported our hypothesis: BMPs can be used for the tratement of the acute radiation sickness. The results indicated that in adult mice , BMPs could recover the hematopoietic injury from acute radiation sickness.更多还原