【摘要】 研制了一种新型的过氧化聚吡咯膜修饰电极（ＯＰＰｙ／ＣＭＥ）作为液相色谱电化学检测器，该电极可以用来检测生物体内的单胺类神经递质及其代谢产物。用药物建立了帕金森动物试验模型，对不同情况下的试验动物脑内单胺类神经递质及其代谢产物通过微渗析取样和液相色谱电化学检测联用技术进行了测定。初步探讨了试验动物产生帕金森病的机理，为研制和筛选更有效的治疗帕金森病的新药提供了一种准确、可靠的分析手段。更多还原

【Abstract】 A novel polymer - overoxidized polypyrrole film chemically modified electrode (CME) as the liquid xhromatography-electrochemical detector (LC-ECD) was fabricated and applicated, which could be used to determine the monoamine neurotrasmitters.The experimental model of parkinsonian animal was established by using medicine.Microdialysis sampling coupled with De-ECD was ed to monitor the monoamine neurotransmitters and their metabolites in experimental animal brain in vivo under different situations. The mechansm of experimental animal’s parkinsonian disease was studied elermentarilh.An accurate and reliable analysis method was supplied for fliting the new and the more effective medicine to cure parkinsonian disease.更多还原