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杂交酸模愈伤组织诱导及植株再生
Studies on Callus Induction,Plant Regenerition and Suspension Culture of Hybrid Slam
【摘要】 杂交酸模是一种多年生、高产优质、抗逆性强的优良牧草资源。本文研究了不同激素组合处理对杂交酸模子叶愈伤组织的诱导、分化和植株再生的影响。实验结果表明,2,4 D配合使用 6 BAP时愈伤组织诱导率最高,诱导芽形成的最佳激素配比为MS +0 5mg/LNAA + 2 0mg/L 6 BAP,诱导根形成的最适培养基为MS + 2 0mg/LIAA,组培苗移栽成活。
【Abstract】 Hybrid slam is a good grass rescource for its high yield,fine quality and tolerant to stress.This paper studied the effects of different hormone combinations and concentrations on callus induction and plant regenerition of hybrid slam.The experimental results showed that the induced rate of callus was the best by MS medium supplemented with 2,4Dand 6 BAP,the best hormone making up of splited bud was MS with 0 5mg/L NAA and 2 0mg/L 6 BAP.Roots appeared on the MS with 2 0mg/L IAA is the best and whole plantlets were formed.
【Key words】 Hybrid Slam; cotyledon; callus induction; plant regenerition;
- 【文献出处】 北京农学院学报 ,Journal of Beijing Agricultural College , 编辑部邮箱 ,2001年04期
- 【分类号】S548
- 【被引频次】2
- 【下载频次】72