【摘要】 用分光光度法研究了桑色素 铝离子 核酸三元体系。在pH 3 2 5的Britton Robinson介质中 ,桑色素在 2 5 0 ,30 0和 35 0nm处有吸收峰。铝离子的加入使桑色素 35 0nm处的吸收峰下降 ,在4 19nm处出现桑色素 铝络合物的吸收峰。再往桑色素 铝离子二元体系中加入核糖核酸或脱氧核糖核酸 ,则进一步引起桑色素 35 0nm吸收峰的降低 ,4 19nm处的吸收大大加强 ,同时在 370nm处有一等色点。 4 19nm处吸光度的增加值与加入的核酸量在一定范围内成正比 ,基于此建立了在较宽范围内测定核酸的方法。其线性范围分别为 :ρ(ctDNA) ,0 71～ 35 4 μg mL ;ρ(fsDNA) ,0 64～ 2 5 6μg mL ;ρ(yRNA) ,0 94～ 2 8 4 μg mL。更多还原

【Abstract】 Ternary system of Morin-Al(Ⅲ)-Nucleic acids was studied by spectrophotometry.At pH 3.25 Britton-Robinson medium,morin has the absorption peaks at 250,300 and 350*!nm.When Al+{3+} was added,the absorption of morin at 350*!nm decreased and a new absorption peak at 419*!nm appeared.Adding of nucleic acids to the morin-Al(Ⅲ) binary system leads to decrease of the absorption at 350*!nm and great increase at 419*!nm.There is an isochromatic point at 370*!nm.The increase of absorbance at 419*!nm is proportional to the amount of nucleic acids added within certain concentration range.Based on this,a new sensitive nucleic acids analysis method is established.The linear ranges of ct DNA,fs DNA and y RNA are 0.71～35.4,0.64～25.6 and 0.94～28.4*!μg/mL,respectively.更多还原